GB 5009.283-2021

Determination of Azodicarbonamide in Food Safety National Standards (English Version)

Standard No.

GB 5009.283-2021

Language

Chinese, Available in English version

Release Date

2021

Published By

National Health Commission of the People's Republic of China

Latest

GB 5009.283-2021

Scope

This standard specifies the liquid chromatographic method for the determination of azodicarbonamide in food. This standard applies to the determination of azodicarbonamide in wheat flour. 2 principles

Introduction

Standard technical framework and core innovation

This standard uses high performance liquid chromatography with triphenylphosphine derivatization method, which improves sensitivity and accuracy compared with traditional methods. The core improvements are reflected in:

Technical indicators	2016 old standard	2021 new standard	Improvement range
Limit of detection (LOD)	5.0 mg/kg	2.0 mg/kg	↓ 60%
Derivatization time	24 hours	≥12 hours	Efficiency↑50%
Instrument applicability	UV detection only	Support DAD detection	Compatibility Extension

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Key Control Points for Experimental Operations

Physical and Chemical Experimental Process

Temperature control is the core element of the derivatization reaction and needs to be controlled at 20 Tracking 20℃ intervals. A recent case from a testing agency showed that if the metabolic byproducts are in a 30℃ environment for more than 18 hours, they will interfere with the effectiveness of the test.

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Quality Control Implementation Specifications

The laboratory quality control system should focus on monitoring:

• Organic microporous membrane adaptation verification (0.22μm accuracy verification)
• N,N-dimethylformamide purity verification plan
• Standard curve linear dynamic range confirmation (R2 ≥0.999)

GB 5009.283-2021 history

• 2021 GB 5009.283-2021 Determination of Azodicarbonamide in Food Safety National Standards

GB 5009.283-2021 -All Parts